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bioRxiv · 10.1101/2022.01.20.477161

Pore-C Simultaneously Captures Genome-wide Multi-way Chromatin Interaction and Associated DNA Methylation Status in Arabidopsis

Li, Z.·Long, Y.·Yu, Y.·Zhang, F.·Zhang, H.·Liu, Z.·Jia, J.·Mo, W.·Tian, S. Z.·Zheng, M.·Zhai, J.
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Abstract

In the past decade, genome-wide characterization of the three-dimensional chromatin structure in plants using high-throughput methods has greatly advanced our knowledge in plant genome architecture (Liu and Weigel, 2015; Ouyang et al., 2020). However, due to the limitation of Illumina short-read sequencing, the genome-wide contact map obtained by Hi-C/ChIA-PET is pairwise, and the multi-way interaction can only be inferred from the two-way data. To directly capture multi-way interaction in Arabidopsis, we applied a long-read-based method called Pore-C that directly sequences the DNA fragments joined by proximity-based ligation (Ulahannan et al., 2019).

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Li, Z., Long, Y., Yu, Y., Zhang, F., Zhang, H., Liu, Z., Jia, J., Mo, W., Tian, S. Z., Zheng, M., Zhai, J.. 2022-01-21. Pore-C Simultaneously Captures Genome-wide Multi-way Chromatin Interaction and Associated DNA Methylation Status in Arabidopsis. https://doi.org/10.1101/2022.01.20.477161

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