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bioRxiv · 10.1101/2022.01.14.473406

Structures of LIG1 engaging with mutagenic mismatches inserted by polβ in base excision repair

Tang, Q.·McKenna, R.·Caglayan, M.
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Abstract

DNA ligase I (LIG1) catalyzes final ligation step following DNA polymerase (pol) {beta} gap filling and an incorrect nucleotide insertion by pol{beta} creates a nick repair intermediate with mismatched end at the downstream steps of base excision repair (BER) pathway. Yet, how LIG1 discriminates against the mutagenic 3'-mismatches at atomic resolution remains undefined. Here, we determined X-ray structures of LIG1/nick DNA complexes with G:T and A:C mismatches and uncovered the ligase strategies that favor or deter ligation of base substitution errors. Our structures revealed that LIG1 active site can accommodate G:T mismatch in a similar conformation with A:T base pairing, while it stays in the LIG1-adenylate intermediate during initial step of ligation reaction in the presence of A:C mismatch at 3'-strand. Moreover, we showed mutagenic ligation and aberrant nick sealing of the nick DNA substrates with 3'-preinserted dG:T and dA:C mismatches, respectively. Finally, we demonstrated that AP-Endonuclease 1 (APE1), as a compensatory proofreading enzyme, interacts and coordinates with LIG1 during mismatch removal and DNA ligation. Our overall findings and ligase/nick DNA structures provide the features of accurate versus mutagenic outcomes at the final BER steps where a multi-protein complex including pol{beta}, LIG1, and APE1 can maintain accurate repair.

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Tang, Q., McKenna, R., Caglayan, M.. 2022-01-14. Structures of LIG1 engaging with mutagenic mismatches inserted by polβ in base excision repair. https://doi.org/10.1101/2022.01.14.473406

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