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bioRxiv · 10.1101/2022.01.11.475855

Rational design yields molecular insights on leaf binding of the anchor peptide Macaque Histatin

Abstract

1In times of a constantly growing world population and increasing demand for food, sustainable agriculture is crucial. To reduce the amount of applied nutrients, herbicides, and fungicides, the rainfastness of plant protection agents is of pivotal importance. As a result of protective agent wash-off, plant protection is lost, and soils and groundwater are severely polluted. To date, rainfastness of plant protection products is achieved by adding polymeric adjuvants to the agrochemicals. However, polymeric adjuvants will be regarded as microplastics in the future, and environmentally friendly alternatives are needed. Anchor peptides (APs) are promising biobased and biodegradable adhesion promoters. While the adhesion of anchor peptides to artificial surfaces, such as polymers, has already been investigated in theory and experimentally, exploiting the adhesion to biological surfaces remains challenging. The complex nature and composition of biological surfaces such as plant leaf and fruit surfaces complicate the generation of accurate models. Here, we present the first detailed three-layered atomistic model of the surface of apple leaves and use it to compute free energy profiles of the adhesion and desorption of APs to and from that surface. Our model is validated by a novel fluorescence-based MTP assay that mimicks these complex processes and allows quantifying them. For the AP Macaque Histatin, we demonstrate that aromatic and positively charged amino acids are essential for binding to the waxy apple leaf surface. The established protocols should generally be applicable for tailoring the binding properties of APs to biological interfaces.

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Dittrich, J., Brethauer, C., Goncharenko, L., Bührmann, J., Zeisler-Diehl, V., Pariyar, S., Jakob, F., Kurkina, T., Schreiber, L., Schwaneberg, U., Gohlke, H.. 2022-01-12. Rational design yields molecular insights on leaf binding of the anchor peptide Macaque Histatin. https://doi.org/10.1101/2022.01.11.475855

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