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bioRxiv · 10.1101/2022.01.10.475704

Spinal cord homeostatic plasticity gates mechanical allodynia in chronic pain

Cao, B.·Scherrer, G.·Chen, L.
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Abstract

Central sensitization caused by disinhibition of spinal cord circuits is a key mechanism of mechanical allodynia in neuropathic pain. Despite intense efforts, the molecular mechanisms that drive disinhibition and induce allodynia after peripheral nerve injury remain unclear. Using the spared-nerve injury (SNI) model of allodynia, we here demonstrate that SNI induces disinhibition of spinal nociceptive circuits by triggering homeostatic synaptic plasticity. Specifically, SNI-triggered homeostatic plasticity suppresses the inhibitory outputs of parvalbumin-positive (PV+) interneurons that form synapses on both primary afferent terminals and excitatory interneurons, causing hyperactivation of the nociceptive pathway. Using genetic manipulations, we identified the retinoic acid receptor RAR as the key mediator of the homeostatic synaptic plasticity underlying this synaptic disinhibition. Deletion of RAR in PV+ neurons blocked SNI-induced spinal disinhibition, central sensitization, and allodynia. Moreover, deletion of RAR in spinal PV+ neurons or application of an RAR antagonist in the spinal cord prevented the development of SNI-induced mechanical allodynia. Together, our results reveal a molecular mechanism of neuropathic pain whereby homeostatic plasticity causes the mis-direction of tactile information flow to ascending nociceptive pathways following peripheral nerve injury.

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Cao, B., Scherrer, G., Chen, L.. 2022-01-12. Spinal cord homeostatic plasticity gates mechanical allodynia in chronic pain. https://doi.org/10.1101/2022.01.10.475704

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