Presence of sodium taurocholate co-transporting polypeptide and Hepatitis B replication marker on placenta: Another home for the virus

Garg, G.; MN, M.; Patel, K.; Purwar, S.; Mukhopadhyay, S.; Mishra, N.; Gupta, S.; Rawat, S. K.; Khosla, R.; Singh, J.; Nema, S.; Biswas, D.; Singh, A. K.; Vyas, A. K.

doi:10.1101/2022.01.05.475013

bioRxiv · 10.1101/2022.01.05.475013

Presence of sodium taurocholate co-transporting polypeptide and Hepatitis B replication marker on placenta: Another home for the virus

Garg, G.·MN, M.·Patel, K.·Purwar, S.·Mukhopadhyay, S.·Mishra, N.·Gupta, S.·Rawat, S. K.·Khosla, R.·Singh, J.·Nema, S.·Biswas, D.·Singh, A. K.·Vyas, A. K.

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Abstract

BackgroundThe role of sodium taurocholate co-transporting polypeptide (NTCP), in facilitating the binding of Hepatitis B virus (HBV) on surface of hepatocytes is well documented. Expression of NTCP in extra hepatic cells may make these cells susceptible to HBV infection and support cellular proliferation akin to hepatocytes. Placental replication of HBV is not well explored. In this study we have assessed the expression of NTCP and HBV replication markers (HBeAg, HBcAg, and HBV DNA) in placental cells, to investigate if these cells act as host for HBV. MethodsFourty one HBsAg+ve pregnant women along with 10 healthy controls were enrolled after obtaining informed consent. The HBV DNA in placenta was detected by qPCR using primers for X and core ORF. Expression of NTCP in placenta was analyzed by qRT-PCR and further investigated by immunohistochemistry (IHC) along with HBV replication biomarkers, HBeAg, and HBcAg. ResultsHBsAg positive subjects were divided in two groups on the basis of viral load [High Viral Load (HVL) Group; viral load [≥] 2000IU/ml, Low Viral Load (LVL) Group; viral load <2000IU/ml] according to INASL guidelines 2018. HBV infected females showed increased expression of NTCP in trophoblasts of placenta compared to control group (HVL 3.69{+/-}0.13 Vs Control 1.74{+/-}0.15, p=0.0117). Furthermore, significant difference in NTCP expression was also observed between HVL and LVL group (HVL 3.69{+/-}0.13 Vs LVL 1.98{+/-}0.17, p=0.022) and positively correlated with the maternal HBV DNA load. Membranous and/or cytoplasmic immunostaining of NTCP, and cytoplasmic staining of HBeAg and HBcAg in trophoblasts along with presence of HBV DNA indicated that trophoblasts are not only susceptible to HBV infection but may also be a site for viral replication. ConclusionsThis is the pioneer study, which demonstrates expression of NTCP on placenta which may facilitate the entry of HBV. Furthermore, the study establishes the presence of HBeAg in placenta of patients without circulating HBeAg, indicating these cells may act as replication host/reservoir. This pioneering finding hints at the possibility of exploring the potential of NTCP blocking strategies in preventing vertical transmission of HBV.

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Garg, G., MN, M., Patel, K., Purwar, S., Mukhopadhyay, S., Mishra, N., Gupta, S., Rawat, S. K., Khosla, R., Singh, J., Nema, S., Biswas, D., Singh, A. K., Vyas, A. K.. 2022-01-05. Presence of sodium taurocholate co-transporting polypeptide and Hepatitis B replication marker on placenta: Another home for the virus. https://doi.org/10.1101/2022.01.05.475013

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