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bioRxiv · 10.1101/2022.01.01.474705

Bacterial composition and inferring function profiles in the biofloc system rearing Litopenaeus vannamei postlarvae at a low salinity

Huang, H.-H.·Li, C.-Y.·Lei, Y.-J.·Kuang, W.-Q.·Zou, W.-S.·Yang, P.-H.
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Abstract

This study aimed to investigate the bacterial composition and inferring function profiles in the biofloc system rearing Litopenaeus vannamei postlarvae (PL) at a low salinity condition. PL (~ stage 15) were stocked in four parallel tanks filled in water with a salinity of 5.0{per thousand} at a density of 4000 individuals per m3 for a 28-days culture experiment, during which glucose was added as carbon source with a C:N of 20:1. At the end of experiment, water was sampled from each tank and pooled to extract microbial DNA for high-throughput sequencing of V3-V4 region of 16S rRNA gene. Results showed that the bacterial community at 28 d was dominated by phyla of Proteobacteria (45.8%), Bacteroidetes (21.1%), Planctomycetes (13.5%), Chlamydiae (10.3%) and Firmicutes (6.8%). A proportion of 81% inferring KEGG functions of this bacterial community associated with metabolism. Among functions relating to nitrogen metabolism, 48.5% were involved in the conversion of ammonia to glutamate, but the proportion of those involved in transformation among ammonia, nitrite and nitrate was 18.0% in total, inferring higher protein-synthesis but lower inorganic nitrogen-transformation capacities of the bacterial community. At the same time (28 d), high levels of total nitrogen (231.3{+/-}6.0 mg L-1) and biofloc (127.0{+/-}63.0 mL L-1), but low concentrations of ammonia (0.04{+/-}0.01 mg L-1), nitrite (0.2{+/-}0.1 mg L-1) and nitrate (12.9{+/-}2.5 mg L-1) were observed. The results supply a novel insight for understanding the function of bacterial community in the biofloc system nursing L. vannamei PL at a low salinity.

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Huang, H.-H., Li, C.-Y., Lei, Y.-J., Kuang, W.-Q., Zou, W.-S., Yang, P.-H.. 2022-01-02. Bacterial composition and inferring function profiles in the biofloc system rearing Litopenaeus vannamei postlarvae at a low salinity. https://doi.org/10.1101/2022.01.01.474705

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