Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex

HATTORI, T.; Cherepanov, S. M.; Sakaga, R.; Roboon, J.; Nguyen, D. T.; Ishii, H.; Takarada-Iemata, M.; Nishiuchi, T.; Kannon, T.; Hosomichi, K.; Tajima, A.; Yamamoto, Y.; Okamoto, H.; Sugawara, A.; Higashida, H.; Hori, O.

doi:10.1101/2021.12.23.474051

bioRxiv · 10.1101/2021.12.23.474051

Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex

HATTORI, T.·Cherepanov, S. M.·Sakaga, R.·Roboon, J.·Nguyen, D. T.·Ishii, H.·Takarada-Iemata, M.·Nishiuchi, T.·Kannon, T.·Hosomichi, K.·Tajima, A.·Yamamoto, Y.·Okamoto, H.·Sugawara, A.·Higashida, H.·Hori, O.

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Abstract

Social behavior is essential for the health, survival and reproduction of animals, yet the role of astrocytes in social behavior is largely unknown. CD38 is critical for social behaviors by regulating oxytocin release from hypothalamic neurons. On the other hand, CD38 is most abundantly expressed in astrocytes especially in the postnatal cortex, and is important for astroglial development. Here, we demonstrate that astroglial CD38 plays a pivotal role in the social behavior. Selective deletion of CD38 in postnatal astrocytes, but not in adult astrocytes, specifically impaired social memory without any other behavioral abnormalities. Morphological analysis revealed reductions in spine numbers, mature spines and excitatory synapse numbers in the pyramidal neurons of the medial prefrontal cortex (mPFC) due to deletion of astroglial CD38 in the postnatal brain. Astrocyte-conditioned medium (ACM) of CD38 KO astrocytes reduced synaptogenesis of cortical neurons by reducing extracellular SPARCL1, a synaptogenic protein. Finally, the release of SPARCL1 from astrocytes is regulated by CD38/cADPR/calcium signaling. Our data indicate that astroglial CD38 developmentally regulates social memory and neural circuit formation in the developing brain by promoting synaptogenesis through SPARCL1.

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HATTORI, T., Cherepanov, S. M., Sakaga, R., Roboon, J., Nguyen, D. T., Ishii, H., Takarada-Iemata, M., Nishiuchi, T., Kannon, T., Hosomichi, K., Tajima, A., Yamamoto, Y., Okamoto, H., Sugawara, A., Higashida, H., Hori, O.. 2021-12-24. Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex. https://doi.org/10.1101/2021.12.23.474051

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