Structure of anchorless RML prion reveals motif variation between strains

Hoyt, F.; Standke, H. G.; Artikis, E.; Schwartz, C. L.; Hansen, B.; Li, K.; Hughson, A. G.; Manca, M.; Thomas, O. R.; Raymond, G. J.; Baron, G. S.; Caughey, B.; Kraus, A.

doi:10.1101/2021.12.22.473909

bioRxiv · 10.1101/2021.12.22.473909

Structure of anchorless RML prion reveals motif variation between strains

Hoyt, F.·Standke, H. G.·Artikis, E.·Schwartz, C. L.·Hansen, B.·Li, K.·Hughson, A. G.·Manca, M.·Thomas, O. R.·Raymond, G. J.·Baron, G. S.·Caughey, B.·Kraus, A.

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Abstract

Little is known about the structural basis of prion strains. Here we provide a high (3.0 [A]) resolution cryo-electron microscopy-based structure of brain-derived fibrils of the mouse anchorless RML scrapie strain which, like the recently determined hamster 263K strain, has a parallel in-register {beta}-sheet-based core. However, detailed comparisons reveal that variations in shared structural motifs provide a basis for prion strain determination. One-sentence summaryCryo-electron microscopy reveals a near-atomic structure of an infectious, brain-derived murine prion fibril and strain differences.

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Hoyt, F., Standke, H. G., Artikis, E., Schwartz, C. L., Hansen, B., Li, K., Hughson, A. G., Manca, M., Thomas, O. R., Raymond, G. J., Baron, G. S., Caughey, B., Kraus, A.. 2021-12-22. Structure of anchorless RML prion reveals motif variation between strains. https://doi.org/10.1101/2021.12.22.473909

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