Insilico and Invitro optimization of Naringin and rutin molecules targeting DNA damage in breast cancer cells

Abstract

Discovering the molecular mechanisms of DNA damage response pathways has led to new therapeutic approaches in oncology. Our study optimized DNA damage-targeting molecules naringin and rutin in breast cancer cells. Our study involved MTT assays for detection of its toxicity and proliferative activity in breast cancer cells and normal cancer cells. Our studies determined the molecules antioxidant properties using the DPPH assay. The role in reducing free radicals has been evaluated using a variety of free radical scavenging activity assays. Further evaluation of the molecules was carried out by high alkaline comet assay (pH >13) to test for genotoxicity. Human Dermal Fibroblast cells (2DD) (1x105 cells/ml) and breast cancer cells (MDA-MB-231) were pre-incubated with Naringin and Rutin (10 {micro}M) for one hour. In normal cells, rutin and naringin molecules do not cause genotoxicity, but they cause DNA damage in breast cancer cells when they are diluted to 10{micro}M. The results from our study indicate that both molecules cause 60-70% DNA damage in breast cancer cells.