Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer

Thiele, J. C.; Jungblut, M.; Helmerich, D. A.; Tsukanov, R.; Chizhik, A.; Chizhik, A. I.; Schnermann, M.; Sauer, M.; Nevskyi, O.; Enderlein, J.

doi:10.1101/2021.12.20.473473

bioRxiv · 10.1101/2021.12.20.473473

Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer

Thiele, J. C.·Jungblut, M.·Helmerich, D. A.·Tsukanov, R.·Chizhik, A.·Chizhik, A. I.·Schnermann, M.·Sauer, M.·Nevskyi, O.·Enderlein, J.

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Abstract

Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously.

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Thiele, J. C., Jungblut, M., Helmerich, D. A., Tsukanov, R., Chizhik, A., Chizhik, A. I., Schnermann, M., Sauer, M., Nevskyi, O., Enderlein, J.. 2021-12-21. Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer. https://doi.org/10.1101/2021.12.20.473473

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