Integrated view and comparative analysis of baseline protein expression in mouse and rat tissues

Wang, S.; Garcia-Seisdedos, D.; Prakash, A.; Kundu, D. J.; Collins, A.; George, N.; Fexova, S.; Moreno, P.; Papatheodorou, I.; Jones, A. R.; Vizcaino, J. A.

doi:10.1101/2021.12.20.473413

bioRxiv · 10.1101/2021.12.20.473413

Integrated view and comparative analysis of baseline protein expression in mouse and rat tissues

Wang, S.·Garcia-Seisdedos, D.·Prakash, A.·Kundu, D. J.·Collins, A.·George, N.·Fexova, S.·Moreno, P.·Papatheodorou, I.·Jones, A. R.·Vizcaino, J. A.

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Abstract

The increasingly large amount of proteomics data in the public domain enables, among other applications, the combined analyses of datasets to create comparative protein expression maps covering different organisms and different biological conditions. Here we have reanalysed public proteomics datasets from mouse and rat tissues (14 and 9 datasets, respectively), to assess baseline protein abundance. Overall, the aggregated dataset contained 23 individual datasets, including a total of 211 samples coming from 34 different tissues across 14 organs, comprising 9 mouse and 3 rat strains, respectively. In all cases, we studied the distribution of canonical proteins between the different organs. The number of canonical proteins per dataset ranged from 273 (tendon) and 9,715 (liver) in mouse, and from 101 (tendon) and 6,130 (kidney) in rat. Then, we studied how protein abundances compared across different datasets and organs for both species. As a key point we carried out a comparative analysis of protein expression between mouse, rat and human tissues. We observed a high level of correlation of protein expression among orthologs between all three species in brain, kidney, heart and liver samples, whereas the correlation of protein expression was generally slightly lower between organs within the same species. Protein expression results have been integrated into the resource Expression Atlas for widespread dissemination. Author summaryWe have reanalysed 23 baseline mass spectrometry-based public proteomics datasets stored in the PRIDE database. Overall, the aggregated dataset contained 211 samples, coming from 34 different tissues across 14 organs, comprising 9 mouse and 3 rat strains, respectively. We analysed the distribution of protein expression across organs in both species. We also studied how protein abundances compared across different datasets and organs for both species. Then we performed gene ontology and pathway enrichment analyses to identify enriched biological processes and pathways across organs. We also carried out a comparative analysis of baseline protein expression across mouse, rat and human tissues, observing a high level of expression correlation among orthologs in all three species, in brain, kidney, heart and liver samples. To disseminate these findings, we have integrated the protein expression results into the resource Expression Atlas.

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Wang, S., Garcia-Seisdedos, D., Prakash, A., Kundu, D. J., Collins, A., George, N., Fexova, S., Moreno, P., Papatheodorou, I., Jones, A. R., Vizcaino, J. A.. 2021-12-21. Integrated view and comparative analysis of baseline protein expression in mouse and rat tissues. https://doi.org/10.1101/2021.12.20.473413

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