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bioRxiv · 10.1101/2021.12.09.471958

Fission of megamitochondria into multiple smaller well-defined mitochondria in the ageing zebrafish retina

Burgoyne, T.·Toms, M.·Way, C.·Tracey-White, D.·Futter, C.·Moosajee, M.
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Abstract

Mitochondria are essential adenosine triphosphate (ATP)-generating cellular organelles. In the retina, they are highly numerous in the photoreceptors and retinal pigment epithelium (RPE) due to their high energetic requirements. Fission and fusion of the mitochondria within these cells allow them to adapt to changing demands over the lifespan of the organism. Using transmission electron microscopy, we examined the mitochondrial ultrastructure of zebrafish photoreceptors and RPE from 5 days post fertilisation (dpf) through to late adulthood (3 years). Notably, mitochondria in the youngest animals were large and irregular shaped with a loose cristae architecture, but by 8 dpf they had reduced in size and expanded in number with more defined cristae. When investigating temporal gene expression of several mitochondrial-related markers, they indicated fission as the dominant mechanism contributing to these changes observed over time. This is likely to be due to continued mitochondrial stress resulting from the oxidative environment of the retina and prolonged light exposure. We have characterised retinal mitochondrial ageing in a key vertebrate model organism, that provides a basis for future studies of retinal diseases that are linked to mitochondrial dysfunction.

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Burgoyne, T., Toms, M., Way, C., Tracey-White, D., Futter, C., Moosajee, M.. 2021-12-10. Fission of megamitochondria into multiple smaller well-defined mitochondria in the ageing zebrafish retina. https://doi.org/10.1101/2021.12.09.471958

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