Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

Abstract

BackgroundTriatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findingsWe used RNA-Seq to explore the early transcriptomic response of T. infestans after intoxication with deltamethrin. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorant-binding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significanceOur work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of genomic information from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore new potential insecticide resistance mechanisms in these insects. Author summaryChagas disease affects millions of people worldwide. In the Southern Cone, the development of pyrethroid resistant populations from T. infestans is related to vector persistence and affects the efficiency of vector control campaigns. Several studies have explored the causes of insecticide resistance in T. infestans populations. However, the global transcriptomic response after insecticide treatment has not been analyzed in this species so far. In this study, we obtained transcriptomic information which allowed us to characterize important gene families despite the absence of genomic information. Furthermore, we performed a quantitative analysis of gene expression after deltamethrin intoxication. The results provided here increase the knowledge on detoxification processes in vectors of Chagas disease, which is essential for the design of new vector control strategies.