Metabolic dysregulation induces impaired lymphocyte memory formation during severe SARS-CoV-2 infection

Abstract

Cellular metabolic dysregulation is a consequence of COVID-19 infection that is a key determinant of disease severity. To understand the mechanisms underlying these cellular changes, we performed high-dimensional immune cell profiling of PBMCs from COVID-19-infected patients, in combination with single cell transcriptomic analysis of COVID-19 BALFs. Hypoxia, a hallmark of COVID-19 ARDS, was found to elicit a global metabolic reprogramming in effector lymphocytes. In response to oxygen and nutrient-deprived microenvironments, these cells shift from aerobic respiration to increase their dependence on anaerobic processes including glycolysis, mitophagy, and glutaminolysis to fulfill their bioenergetic demands. We also demonstrate metabolic dysregulation of ciliated lung epithelial cells is linked to significant increase of proinflammatory cytokine secretion and upregulation of HLA class 1 machinery. Augmented HLA class-1 antigen stimulation by epithelial cells leads to cellular exhaustion of metabolically dysregulated CD8 and NK cells, impairing their memory cell differentiation. Unsupervised clustering techniques revealed multiple distinct, differentially abundant CD8 and NK memory cell states that are marked by high glycolytic flux, mitochondrial dysfunction, and cellular exhaustion, further highlighting the connection between disrupted metabolism and impaired memory cell function in COVID-19. Our findings provide novel insight on how SARS-CoV-2 infection affects host immunometabolism and anti-viral response during COVID-19. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=85 SRC="FIGDIR/small/471421v1_ufig1.gif" ALT="Figure 1"> View larger version (32K): org.highwire.dtl.DTLVardef@18bff7borg.highwire.dtl.DTLVardef@31f46borg.highwire.dtl.DTLVardef@1a5ad50org.highwire.dtl.DTLVardef@1577a0_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LIHypoxia and anaerobic glycolysis drive CD8, NK, NKT dysfunction C_LIO_LIHypoxia and anaerobic glycolysis impair memory differentiation in CD8 and NK cells C_LIO_LIHypoxia and anaerobic glycolysis cause mitochondrial dysfunction in CD8, NK, NKT cells C_LI