Differential EDS1 requirement for cell death activities of plant TIR-domain proteins

Lapin, D.; Johanndrees, O.; Baggs, E.; Uhlmann, C.; Locci, F.; Laessle, H. L.; Melkonian, K.; Kaeufer, K.; Dongus, J. A.; Nakagami, H.; Krasileva, K. V.; Parker, J. E.

doi:10.1101/2021.11.29.470438

bioRxiv · 10.1101/2021.11.29.470438

Differential EDS1 requirement for cell death activities of plant TIR-domain proteins

Lapin, D.·Johanndrees, O.·Baggs, E.·Uhlmann, C.·Locci, F.·Laessle, H. L.·Melkonian, K.·Kaeufer, K.·Dongus, J. A.·Nakagami, H.·Krasileva, K. V.·Parker, J. E.

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Abstract

Toll/interleukin-1 Receptor (TIR) domains are integral to immune systems across all domains of life. TIRs exist as single-domain and as larger receptor or adaptor proteins. In plants, TIRs constitute N-terminal domains of nucleotide-binding leucine-rich repeat (NLR) immune receptors. Although TIR-NLR and TIR signaling requires the Enhanced disease susceptibility 1 (EDS1) protein family, TIR domains persist in species that have incomplete or no EDS1 members. To assess whether particular TIR groups appear with EDS1, we searched for TIR-EDS1 co-occurrence patterns. Using a large-scale phylogenetic analysis of TIR domains from 39 algae and land plant species, we identify four conserved TIR groups, two of which are TIR-NLRs present in eudicots and two are more widespread. Presence of one TIR-only protein group is highly correlated with EDS1 and members of this group elicit EDS1-dependent cell death. By contrast, a more widely represented TIR group of TIR-NB-WD40/TPR (TNP) proteins (formerly called XTNX) has at least one member which can induce EDS1-independent cell death. Our data provide a new phylogeny-based plant TIR classification and identify TIR groups that appear to have evolved with and are dependent on EDS1, while others have EDS1-independent activity. One sentence summaryLand plants have evolved four conserved TIR groups

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Lapin, D., Johanndrees, O., Baggs, E., Uhlmann, C., Locci, F., Laessle, H. L., Melkonian, K., Kaeufer, K., Dongus, J. A., Nakagami, H., Krasileva, K. V., Parker, J. E.. 2021-11-30. Differential EDS1 requirement for cell death activities of plant TIR-domain proteins. https://doi.org/10.1101/2021.11.29.470438

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