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bioRxiv · 10.1101/2021.11.16.468655

Rapid nuclear deadenylation of mammalian messenger RNA

Alles, J.·Legnini, I.·Pacelli, M.·Rajewsky, N.
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Abstract

Poly(A) tails protect RNAs from degradation and their deadenylation rates determine RNA stability. Although poly(A) tails are generated in the nucleus, deadenylation of tails has mostly been investigated within the cytoplasm. Here, we combined long-read sequencing with metabolic labeling, splicing inhibition, and cell fractionation experiments to quantify, separately, the genesis and trimming of nuclear and cytoplasmic tails in vitro and in vivo. We present evidence for genome-wide, nuclear synthesis of tails longer than 200 nt, which are rapidly shortened within minutes after transcription. Our data show that rapid deadenylation is a nuclear process, and that different classes of transcripts and even transcript isoforms have distinct nuclear tail lengths. For example, many long-noncoding RNAs escape rapid nuclear deadenylation. Modelling deadenylation dynamics predicts nuclear deadenylation about 10 times faster than cytoplasmic deadenylation. In summary, our data suggest that nuclear deadenylation is a key mechanism for regulating mRNA stability, abundance, and subcellular localization.

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Alles, J., Legnini, I., Pacelli, M., Rajewsky, N.. 2021-11-18. Rapid nuclear deadenylation of mammalian messenger RNA. https://doi.org/10.1101/2021.11.16.468655

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