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bioRxiv · 10.1101/2021.11.14.468515

CXCR4+ Treg cells control serum IgM levels and natural IgM autoantibody production by B1 cells in the bone marrow

Elias, S.·Sharma, R.·Schizas, M.·Valdez, I.·Park, S.-M.·Gonzalez-Figueroa, P.·Li, Q.-Z.·Hoyos, B.·Rudensky, A. Y.
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Abstract

Regulatory T (Treg) cells represent a specialized lineage of suppressive CD4+ T cells, whose functionality is critically dependent on their ability to migrate to, and dwell in the proximity of cells they control. Here we show that continuous expression of the chemokine receptor CXCR4 in Treg cells is required for their ability to accumulate in the bone marrow (BM). Induced CXCR4 ablation in Treg cells led to their rapid depletion and consequent increase in mature B cells, foremost B-1 subset, observed exclusively in the BM without detectable changes in plasma cells or hematopoietic stem cells or any signs of systemic or local immune activation elsewhere. Dysregulation of BM B-1 B cells was associated with a highly specific increase in IgM autoantibodies and the total serum IgM levels. Thus, Treg cells control autoreactive B-1 B cells in a CXCR4-dependent manner. These findings have significant implications for understanding of regulation of B cell autoreactivity and malignancies.

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Elias, S., Sharma, R., Schizas, M., Valdez, I., Park, S.-M., Gonzalez-Figueroa, P., Li, Q.-Z., Hoyos, B., Rudensky, A. Y.. 2021-11-14. CXCR4+ Treg cells control serum IgM levels and natural IgM autoantibody production by B1 cells in the bone marrow. https://doi.org/10.1101/2021.11.14.468515

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