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bioRxiv · 10.1101/2021.11.14.468503

Global landscape of replicative DNA polymerase usage in the human genome

Koyanagi, E.·Kakimoto, Y.·Yoshifuji, F.·Natsume, T.·Higashitani, A.·Ogi, T.·Carr, A. M.·Kanemaki, M. T.·Daigaku, Y.
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Abstract

The division of labour among DNA polymerase underlies the accuracy and efficiency of replication. However, the roles of replicative polymerases have not been directly established in human cells. We developed polymerase usage sequence (Pu-seq) in HCT116 cells and mapped Pol{varepsilon} and Pol usage genome wide. The polymerase usage profiles show Pol{varepsilon} synthesises the leading strand and Pol contributes mainly to lagging strand synthesis. Combining the Pol{varepsilon} and Pol profiles, we accurately predict the genome-wide pattern of fork directionality plus zones of replication initiation and termination. We confirm that transcriptional activity contributes to the pattern of initiation and termination and, by separately analysing the effect of transcription on both co-directional and converging forks, demonstrate that coupled DNA synthesis of leading and lagging strands in both co- directional and convergent forks is compromised by transcription. Polymerase uncoupling is particularly evident in the vicinity of large genes, including the two most unstable common fragile sites, FRA3B and FRA3D, thus linking transcription-induced polymerase uncoupling to chromosomal instability.

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Koyanagi, E., Kakimoto, Y., Yoshifuji, F., Natsume, T., Higashitani, A., Ogi, T., Carr, A. M., Kanemaki, M. T., Daigaku, Y.. 2021-11-14. Global landscape of replicative DNA polymerase usage in the human genome. https://doi.org/10.1101/2021.11.14.468503

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