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bioRxiv · 10.1101/2021.11.09.467982

DNA Self-Assembled Plasmonic Nanodiamonds for Biological Sensing

Liang, L.·Zheng, P.·Jia, S.·Ray, K.·Chen, Y.·Barman, I.
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Abstract

Nitrogen-vacancy (NV) centers in diamonds are promising solid-state quantum emitters for developing superior biological imaging modalities. They possess desired bio-compatibility, photostability and electronic spin-related photophysical properties that are optically accessible at room temperature. Yet, bare nanodiamond-based imaging modalities are limited by the brightness and temporal resolution due to the intrinsically long lifetime of NV centers. Moreover, it remains a technological challenge using top-down fabrication to create freestanding hybrid nanodiamond imaging probes with enhanced performance. In this study, we leverage the bottom-up DNA self-assembly to develop a hybrid plasmonic nanodiamond construct, which we coin as the plasmon-enhanced nanodiamond (PEN), for biological imaging. The PEN nano-assembly features a closed plasmonic nanocavity that completely encapsulates a single nanodiamond, thus enabling the largest possible plasmonic enhancement to accelerate the emission dynamics of NV centers. Creation of the PEN nano-assembly is size-independent, so is its broadband scattering spectrum that is optimally overlapped with the emission spectrum of NV centers. Study of the structure-property correlation reveals that the optimal condition for emission dynamics modification is causally linked to that for a plasmonic nanocavity. The cellular internalization and cytotoxicity studies further confirm the delivery efficiency and biological safety of PEN nano-assemblies. Collectively, the PEN nano-assembly provides a promising approach for manipulating photophysical properties of solid-state quantum emitters and could serve as a versatile platform to uncover non-trivial quantum effects in biological systems.

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Liang, L., Zheng, P., Jia, S., Ray, K., Chen, Y., Barman, I.. 2021-11-11. DNA Self-Assembled Plasmonic Nanodiamonds for Biological Sensing. https://doi.org/10.1101/2021.11.09.467982

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