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bioRxiv · 10.1101/2021.11.08.467288

A full-length infectious cDNA clone of a dsRNA totivirus-like virus

Wang, H.·Okamoto, K.
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Abstract

Totivirus-like viruses are icosahedral non-enveloped double-stranded (ds)RNA viruses belonging to a group recently discovered and provisionally assigned in the Totiviridae family. Unlike fungal and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first totivirus-like virus Omono River virus (OmRV) infectious full-length DNA clone and produce the infectious particles using an RNA-transcript-based method. Unlike the parent wild-type particles from nature, the reverse-genetically-generated OmRV particles had an indistinguishable cytopathic effect, infectivity, and morphology. The established system is one of the few systems that have been reported for generating a non-segmented dsRNA virus DNA clone.

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Wang, H., Okamoto, K.. 2021-11-08. A full-length infectious cDNA clone of a dsRNA totivirus-like virus. https://doi.org/10.1101/2021.11.08.467288

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