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bioRxiv · 10.1101/2021.11.05.467415

Shank promotes action potential repolarization by recruiting BK channels to calcium nanodomains

Gao, L.·Zhao, J.·Ardiel, E. L.·Hall, Q.·Nurrish, S.·Kaplan, J. M.
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Abstract

Mutations altering the scaffolding protein Shank are linked to several psychiatric disorders, and to synaptic and behavioral defects in mice. Among its many binding partners, Shank directly binds CaV1 voltage activated calcium channels. Here we show that the C. elegans SHN-1/Shank promotes CaV1 coupling to calcium activated potassium channels. Mutations inactivating SHN-1, and those preventing SHN-1 binding to EGL-19/CaV1 all increase action potential durations in body muscles. Action potential repolarization is mediated by two classes of potassium channels: SHK-1/KCNA and SLO-1 and SLO-2 BK channels. BK channels are calcium-dependent, and their activation requires tight coupling to EGL-19/CaV1 channels. SHN-1s effects on AP duration are mediated by changes in BK channels. In shn-1 mutants, SLO-2 currents and channel clustering are significantly decreased in both body muscles and neurons. Finally, increased and decreased shn-1 gene copy number produce similar changes in AP width and SLO-2 current. Collectively, these results suggest that an important function of Shank is to promote nanodomain coupling of BK with CaV1.

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Gao, L., Zhao, J., Ardiel, E. L., Hall, Q., Nurrish, S., Kaplan, J. M.. 2021-11-05. Shank promotes action potential repolarization by recruiting BK channels to calcium nanodomains. https://doi.org/10.1101/2021.11.05.467415

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