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bioRxiv · 10.1101/2021.11.04.467378

A high-throughput, automated, cell-free expression and screening platform for antibody discovery

Hunt, A. C.·Vogeli, B.·Kightlinger, W. K.·Yoesep, D. J.·Kruger, A.·Jewett, M. C.
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Abstract

Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific hits. Here, we address this gap by developing an automated workflow combining cell-free DNA template generation, protein synthesis, and high-throughput binding measurements of antibody fragments in a process that takes hours rather than weeks. We apply this workflow to 119 published SARS-CoV-2 neutralizing antibodies and demonstrate rapid identification of the most potent antibody candidates.

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Hunt, A. C., Vogeli, B., Kightlinger, W. K., Yoesep, D. J., Kruger, A., Jewett, M. C.. 2021-11-05. A high-throughput, automated, cell-free expression and screening platform for antibody discovery. https://doi.org/10.1101/2021.11.04.467378

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