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bioRxiv · 10.1101/2021.11.04.467245

CDK1 and PLK1 cooperate to regulate BICD2, dynein activation and recruitment to the nucleus as well as centrosome separation in G2/M

Gallisa-Sune, N.·Sanchez-Fernandez-de-Landa, P.·Zimmermann, F.·Serna, M.·Paz, J.·Regue, L.·Llorca, O.·Luders, J.·Roig, J.
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Abstract

The activity of dynein is regulated by a number of adaptors that mediate its interaction with dynactin, effectively activating the motor complex while also connecting it to different cargos. The regulation of adaptors is consequently central to dynein physiology, but remains largely unexplored. We now describe that one of the best-known dynein adaptors, BICD2, is effectively activated through phosphorylation. In G2 phosphorylation of BICD2 by CDK1 promotes its interaction with PLK1. In turn, PLK1 phosphorylation of a single residue in the N-terminus of BICD2 results in a conformational change that facilitates interaction with dynein and dynactin, allowing the formation of active motor complexes. BICD2 phosphorylation is central for dynein recruitment to the nuclear envelope, centrosome tethering to the nucleus and centrosome separation in G2/M. This work reveals adaptor activation through phosphorylation as crucial for the spatiotemporal regulation of dynein activity.

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Gallisa-Sune, N., Sanchez-Fernandez-de-Landa, P., Zimmermann, F., Serna, M., Paz, J., Regue, L., Llorca, O., Luders, J., Roig, J.. 2021-11-04. CDK1 and PLK1 cooperate to regulate BICD2, dynein activation and recruitment to the nucleus as well as centrosome separation in G2/M. https://doi.org/10.1101/2021.11.04.467245

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