A high-fat diet changes astrocytic metabolism to enhance synaptic plasticity and promote exploratory behavior

Popov, A.; Brazhe, N.; Fedotova, A.; Tiaglik, A.; Bychkov, M.; Morozova, K.; Brazhe, A.; Aronov, D.; Lyukmanova, E.; Lazareva, N.; Li, L.; Verkhratsky, A.; Semyanov, A.

doi:10.1101/2021.11.03.467141

bioRxiv · 10.1101/2021.11.03.467141

A high-fat diet changes astrocytic metabolism to enhance synaptic plasticity and promote exploratory behavior

Popov, A.·Brazhe, N.·Fedotova, A.·Tiaglik, A.·Bychkov, M.·Morozova, K.·Brazhe, A.·Aronov, D.·Lyukmanova, E.·Lazareva, N.·Li, L.·Verkhratsky, A.·Semyanov, A.

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Abstract

A high-fat diet (HFD) is generally considered to negatively influence the body, the brain, and cognitive abilities. On the other hand, fat and fatty acids are essential for nourishing and constructing brain tissue. Astrocytes are central for lipolysis and fatty acids metabolism. Here we show that exposure of young mice to one month of HFD elevates lipid content and increases the relative amount of reduced cytochromes in astrocytes but not in neurons. Metabolic changes were paralleled with an enlargement of astrocytic territorial domains due to an increased outgrowth of branches and leaflets. Astrocyte remodeling was associated with an increase in expression of ezrin and with no changes in glial fibrillary acidic protein (GFAP), glutamate transporter-1 (GLT-1), and glutamine synthetase (GS). Such physiological (non-reactive) enlargement of astrocytes in the brain active milieu promoted glutamate clearance and long-term potentiation. These changes translated into improved exploratory behavior. Thus, dietary fat intake is not invariably harmful and might exert beneficial effects depending on the biological context. In BriefA high-fat diet stimulates the metabolism and growth of astrocytes, which improves glutamate clearance, synaptic plasticity, and exploratory behavior in young mice. Thus, dietary fat arguably is an essential component of the diet for children and young adults, supporting the optimal development of the brain. HighlightsO_LIExposure of young mice to a high-fat diet elevated lipid content and increased amount of reduced cytochromes in astrocytes but not in neurons. C_LIO_LIMetabolic changes were paralleled with an enlargement of astrocytic territorial domains due to an increased outgrowth of branches and leaflets. C_LIO_LIAstrocytic enlargement was associated with increased expression of ezrin but not GFAP, hence was not reactive but physiological C_LIO_LIExpansion of astrocytes in the brain active milieu improved glutamate clearance and long-term potentiation. C_LIO_LIThe high-fat diet improved exploratory behavior in young mice. C_LI

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Popov, A., Brazhe, N., Fedotova, A., Tiaglik, A., Bychkov, M., Morozova, K., Brazhe, A., Aronov, D., Lyukmanova, E., Lazareva, N., Li, L., Verkhratsky, A., Semyanov, A.. 2021-11-04. A high-fat diet changes astrocytic metabolism to enhance synaptic plasticity and promote exploratory behavior. https://doi.org/10.1101/2021.11.03.467141

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