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bioRxiv · 10.1101/2021.10.26.465876

Eukaryotic CRFK cells motion characterized with atomic force microscopy

Zamora-Ceballos, M.·Barcena, J.·Mertens, J.
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Abstract

We performed a time-lapse imaging with Atomic Force Microscopy (AFM) of the motion of eukaryotic CRFK (Crandell-Rees Feline Kidney) cells adhered onto a glass surface and anchored to other cells in culture medium at 37{degrees}C. The main finding is a gradient in the spring constant of the actomyosin cortex along the cells axis. The rigidity increases at the rear of the cells during motion. This observation as well as a dramatic decrease of the volume suggests that cells may organize a dissymmetry in the skeleton network to expulse water and drive actively the rear edge.

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Zamora-Ceballos, M., Barcena, J., Mertens, J.. 2021-10-26. Eukaryotic CRFK cells motion characterized with atomic force microscopy. https://doi.org/10.1101/2021.10.26.465876

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