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bioRxiv · 10.1101/2021.10.21.465351

Kinetic principles underlying pioneer function of GAGA transcription factor in live cells

Wu, C.·Tang, X.·Li, T.·Liu, S.·Wisniewski, J.·Zheng, Q.·Lavis, L.·Rong, Y.
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Abstract

How pioneer factors interface with chromatin to promote accessibility for transcription control is poorly understood in vivo. Here, we directly visualize chromatin association by the prototypical GAGA pioneer factor (GAF) in live Drosophila hemocytes. Single-particle tracking reveals that the majority of GAF is chromatin-bound, with a stable-binding fraction showing nucleosome-like confinement residing on chromatin for over 2 minutes, far longer than the dynamic range of most transcription factors. These kinetic properties require the full complement of GAFs DNA-binding, multimerization and intrinsically disordered domains, and are autonomous from recruited chromatin remodelers NURF and PBAP, whose activities primarily benefit GAFs neighbors such as HSF. Evaluation of GAF kinetics together with its endogenous abundance indicates that despite on-off dynamics, GAF constitutively and fully occupies chromatin targets, thereby providing a temporal mechanism that sustains open chromatin for transcriptional responses to homeostatic, environmental, and developmental signals.

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Wu, C., Tang, X., Li, T., Liu, S., Wisniewski, J., Zheng, Q., Lavis, L., Rong, Y.. 2021-10-23. Kinetic principles underlying pioneer function of GAGA transcription factor in live cells. https://doi.org/10.1101/2021.10.21.465351

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