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bioRxiv · 10.1101/2021.10.20.465128

Identifying the oncogenic potential of gene fusions exploiting miRNAs

Lovino, M.·Montemurro, M.·Barrese, V. S.·Ficarra, E.
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Abstract

BackgroundIt is estimated that oncogenic gene fusions cause about 20% of human cancer morbidity. Identifying potentially oncogenic gene fusions may improve affected patients diagnosis and treatment. Previous approaches to this issue included exploiting specific gene-related information, such as gene function and regulation. Here we propose a model that profits from the previous findings and includes the microRNAs in the oncogenic assessment. We present a classifier called ChimerDriver for the classification of gene fusions as oncogenic or not oncogenic. ChimerDriver is based on a specifically designed neural network and trained on genetic and post-transcriptional information to obtain a reliable classification. Results and discussionThe designed neural network integrates information related to transcription factors, gene ontologies, microRNAs and other detailed information related to the functions of the genes involved in the fusion and the gene fusion structure. As a result, the performances on the test set reached 0.83 f1-score and 96% recall. The comparison with state-of-the-art tools returned comparable or higher results. Moreover, ChimerDriver performed well in a real-world case where 21 out of 24 validated gene fusion samples were detected by the gene fusion detection tool Starfusion. ConclusionsChimerDriver integrated transcriptional and post-transcriptional information in an ad-hoc designed neural network to effectively discriminate oncogenic gene fusions from passenger ones.

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Lovino, M., Montemurro, M., Barrese, V. S., Ficarra, E.. 2021-10-20. Identifying the oncogenic potential of gene fusions exploiting miRNAs. https://doi.org/10.1101/2021.10.20.465128

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