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bioRxiv · 10.1101/2021.10.05.462725

LGG-1/GABARAP lipidation is dispensable for autophagy and development in C .elegans

Abstract

The ubiquitin-like proteins Atg8/LC3/GABARAP are required for multiple steps of autophagy such as initiation, cargo recognition and engulfment, vesicle closure and degradation. Most of LC3/GABARAP functions are considered dependent on their post-translational modifications and addressing to membranes through a conjugation to a lipid, the phosphatidylethanolamine. Contrarily to mammals, C. elegans possesses single homologs of LC3 and GABARAP families, named LGG-2 and LGG-1. Using site directed mutagenesis, we inhibited the conjugation of LGG-1 to the autophagosomal membrane and generated mutants that express only cytosolic forms, either the precursor or the cleaved protein. LGG-1 is an essential gene for autophagy and development in C. elegans, but we discovered that its functions could be fully achieved independently of its localization to the membrane. This study reveals an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy independent embryonic function. Our data question the use of the lipidated GABARAP/LC3 as the main marker of autophagic flux and highlight the high plasticity of autophagy.

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BibTeXRIS

Leboutet, R., Largeau, C., Prigent, M., Quinet, G., Rodriguez, M. S., Cuif, M.-H., Culetto, E., Lefebvre, C., Legouis, R.. 2021-10-05. LGG-1/GABARAP lipidation is dispensable for autophagy and development in C .elegans. https://doi.org/10.1101/2021.10.05.462725

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