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bioRxiv · 10.1101/2021.10.01.462690

Topographic connectivity and cellular profiling reveal detailed input pathways and functionally distinct cell types in the subthalamic nucleus

Jeon, H.·Lee, H.·Kwon, D.-H.·Kim, J.·Tanaka-Yamamoto, K.·Feng, L.·Park, H.·Lim, Y. H.·Cho, Z.-H.·Paek, S. H.
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Abstract

The subthalamic nucleus (STN) controls psychomotor activity and is an efficient therapeutic deep brain stimulation target in Parkinsons disease patients. Despite evidence indicating position-dependent therapeutic effects and distinct functions within the STN, input circuit and cellular profile in the STN remain largely unclear. Using advanced neuroanatomical techniques, we constructed a comprehensive connectivity map of the indirect and hyperdirect pathways in both the mouse and human STN. Our detailed circuit- and cellular-level connectivity revealed a topographically graded organization with three convergent types of indirect and hyperdirect-pathways. Furthermore, we identified two functional types of glutamatergic STN neurons (parvalbumin, PV +/- neurons) segregated with a topographical distribution. Glutamatergic PV+ STN neurons contribute to burst firing. We confirmed synaptic connectivity from indirect and hyperdirect pathways to both PV+ and PV-. These data suggest a complex interplay of information integration within the basal ganglia underlying coordinated movement control and therapeutic effects.

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Jeon, H., Lee, H., Kwon, D.-H., Kim, J., Tanaka-Yamamoto, K., Feng, L., Park, H., Lim, Y. H., Cho, Z.-H., Paek, S. H.. 2021-10-01. Topographic connectivity and cellular profiling reveal detailed input pathways and functionally distinct cell types in the subthalamic nucleus. https://doi.org/10.1101/2021.10.01.462690

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