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bioRxiv · 10.1101/2021.09.22.461420

Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing

Chattopadhyay, S.·Teixeira, L. B. C.·Kiessling, L. L.·McAnulty, J. F.·Raines, R. T.
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Abstract

Transforming growth factor-{beta} (TGF-{beta}) plays important roles in wound healing. The activity of TGF-{beta} is initiated upon binding of the growth factor to extracellular domains of its receptors. We sought to facilitate activation by clustering these extracellular domains. To do so, we used a known peptide that binds to TGF-{beta} receptors without diminishing their affinity for TGF-{beta}. We conjugated this peptide to a collagen-mimetic peptide that can anneal to damaged collagen in a wound bed. We find that the conjugate enhances collagen deposition and wound closure in mice in a manner consistent with the clustering of TGF-{beta} receptors. This strategy provides a means to upregulate the TGF-{beta} signaling pathway without adding exogenous TGF-{beta} and could inspire means to treat severe wounds. TOC Graphic O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=107 SRC="FIGDIR/small/461420v1_ufig1.gif" ALT="Figure 1"> View larger version (52K): org.highwire.dtl.DTLVardef@a0c248org.highwire.dtl.DTLVardef@332b3forg.highwire.dtl.DTLVardef@15c3533org.highwire.dtl.DTLVardef@a8b75a_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Chattopadhyay, S., Teixeira, L. B. C., Kiessling, L. L., McAnulty, J. F., Raines, R. T.. 2021-09-23. Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing. https://doi.org/10.1101/2021.09.22.461420

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