In vivo intraoral waterflow quantification reveals hidden mechanisms of suction feeding in fish

Abstract

Virtually all fish rely on flows of water to transport food to the back of their pharynx. While external flows that draw food into the mouth are well described, how intra-oral water flows manage to deposit food at the esophagus entrance remains unknown. In theory, the posteriorly moving water must, at some point, curve laterally and/or ventrally to exit through the gill slits. Such flows would eventually carry food away from the esophagus instead of towards it. This apparent paradox calls for a filtration mechanism to deviate food from the suction-feeding streamlines. To study this gap in our fundamental understanding of how fish feed, we developed and applied a new technique to quantify three-dimensional patterns of intra-oral water flows in vivo. We combined stereoscopic high-speed x-ray videos to quantify skeletal motion (XROMM) with 3D x-ray particle tracking (XPT) of approximately neutrally buoyant spheres of 1.4 mm in diameter. We showed, for carp (Cyprinus carpio) and tilapia (Oreochromis niloticus), that water tracers displayed higher curvatures than food tracers, indicating an inertia-driven filtration. In addition, tilapia also exhibited a central jet flow pattern, which aids in quickly carrying food to the pharyngeal jaw region. When the food was trapped at the branchial basket, it was resuspended and carried more centrally by periodical bidirectional waterflows, synchronized with head-bone motions. By providing a complete picture of the suction-feeding process and revealing fundamental differences in food transport mechanisms among species, this new technique opens a new area of investigation to fully understand how most aquatic vertebrates feed.