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bioRxiv · 10.1101/2021.09.15.460503

Effect of sodium silicate on drinking water biofilm development

Munoz, S.·Trueman, B. F.·Li, B.·Gagnon, G. A.
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Abstract

Sodium silicates have been studied for sequestration of iron, coagulation, and corrosion control, but their impact on biofilm formation has not been documented in detail. This study investigated the impact of sodium silicate corrosion control on biomass accumulation in drinking water systems in comparison to orthophosphate, a common corrosion inhibitor. Biofilm growth was measured by determining ATP concentrations, and the bacterial community was characterized using 16S ribosomal RNA (rRNA) sequencing. A pilot-scale study with cast-iron pipe loops, annular reactors (ARs), and polycarbonate coupons demonstrated significantly lower biofilm ATP concentrations in the sodium silicate-treated AR than the orthophosphate-treated AR when the water temperature exceeded 20{degrees}C. However, an elevated sodium silicate dose (48 mg L-1 of SiO2) disturbed and dispersed the biofilm formed inside the AR, resulting in elevated effluent ATP concentrations. Two separate experiments confirmed that biomass accumulation was higher in the presence of orthophosphate at high water temperatures (20{degrees}C) only. No significant differences were identified in biofilm ATP concentrations at lower water temperatures (below 20{degrees}C). Differences in bacterial communities between the orthophosphate- and sodium silicate-treated systems were not statistically significant, even though orthophosphate promoted higher biofilm growth. However, the genera Halomonas and Mycobacterium--which include opportunistic pathogens--were present at greater relative abundances in the orthophosphate-treated system compared to the sodium silicate system. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=111 SRC="FIGDIR/small/460503v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@14ac86corg.highwire.dtl.DTLVardef@ac500borg.highwire.dtl.DTLVardef@bb2980org.highwire.dtl.DTLVardef@1a1b5c0_HPS_FORMAT_FIGEXP M_FIG C_FIG Orthophosphate promotes more biofilm growth in comparison to sodium silicates at water temperatures above 20{degrees}C. Water impact statementSodium silicates have been used in drinking water treatment for decades, both as sequestrants and as corrosion inhibitors. However, their impact on biofilm formation is poorly understood, and this risks drinking water quality. This study aims to further clarify the effects of corrosion inhibitors on biofilm development, including inhibitors that are not phosphate-based.

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Munoz, S., Trueman, B. F., Li, B., Gagnon, G. A.. 2021-09-15. Effect of sodium silicate on drinking water biofilm development. https://doi.org/10.1101/2021.09.15.460503

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