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bioRxiv · 10.1101/2021.09.13.460128

Decoding the olfactory map: targeted transcriptomics link olfactory sensory neurons to glomeruli

Zhu, K. W.·Burton, S. D.·Nagai, M. H.·Silverman, J. D.·de March, C. A.·Wachowiak, M.·Matsunami, H.
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Abstract

Sensory processing in vertebrate olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli. Here we combined spatial sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position. ONE SENTENCE SUMMARYTargeted enrichment of olfactory receptor mRNA in olfactory bulb sections determines spatial positions for murine glomeruli.

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Zhu, K. W., Burton, S. D., Nagai, M. H., Silverman, J. D., de March, C. A., Wachowiak, M., Matsunami, H.. 2021-09-14. Decoding the olfactory map: targeted transcriptomics link olfactory sensory neurons to glomeruli. https://doi.org/10.1101/2021.09.13.460128

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