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bioRxiv · 10.1101/2021.09.08.459459

Functional characterization of the biogenic amine transporter system on human macrophages

Abstract

Monocyte-derived macrophages are key players in tissue homeostasis and disease regulated by a variety of signaling molecules. Recent literature has highlighted the ability for biogenic amines to regulate macrophage functions, but the mechanisms governing biogenic amine signaling on and around immune cells remains nebulous. In the central nervous system, biogenic amine transporters are regarded as the master regulators of neurotransmitter signaling. While we and others have shown macrophages express these transporters, relatively little is known of their function on these cells. To address these knowledge gaps, we interrogated the function of norepinephrine (NET) and dopamine (DAT) transporters on human monocyte-derived macrophages. We found that both NET and DAT are present and can uptake substrate from the extracellular space at baseline. Not only was DAT expressed in cultured macrophages, but it was also detected in a subset of intestinal macrophages in situ. Surprisingly, we discovered a NET-independent, DAT-mediated immuno-modulatory mechanism in response to lipopolysaccharide (LPS). LPS induced reverse transport of dopamine through DAT, engaging autocrine/paracrine signaling loop that regulated the macrophage response. Removing this signaling loop enhanced the pro-inflammatory response to LPS. Finally, we found that this DAT-immune axis was disrupted in disease. Collectively, our data introduce a novel role for DAT in the regulation of innate immunity during health and disease.

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BibTeXRIS

Mackie, P. M., Gopinath, A., Montas, D. M., Nielsen, A., Nolan, R., Runner, K., Matt, S., McNamee, J., Ricklan, J., Adachi, K., Doty, A., Ramirez-Zamora, A., Yan, L., Gaskill, P. J., Streit, W. J., Okun, M. S., Khoshbouei, H.. 2021-09-09. Functional characterization of the biogenic amine transporter system on human macrophages. https://doi.org/10.1101/2021.09.08.459459

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