The effect of substrate stiffness on tensile force transduction in the epithelial monolayers

Abstract

In recent years, the importance of mechanical signaling and the cellular mechanical microenvironment in affecting cellular behavior has been widely accepted. Cells in epithelial monolayers are mechanically connected to each other and the underlying extracellular matrix (ECM), forming a highly connected mechanical system subjected to various mechanical cues from their environment, such as the ECM stiffness. Changes in the ECM stiffness have been linked to many pathologies, including tumor formation. However, our understanding of how ECM stiffness and its heterogeneities affect the transduction of mechanical forces in epithelial monolayers is lacking. To investigate this, we used a combination of experimental and computational methods. The experiments were conducted using epithelial cells cultured on an elastic substrate and applying a mechanical stimulus by moving a single cell by micromanipulation. To replicate our experiments computationally and quantify the forces transduced in the epithelium, we developed a new model that described the mechanics of both the cells and the substrate. Our model further enabled the simulations with local stiffness heterogeneities. We found the substrate stiffness to distinctly affect the force transduction as well as the cellular movement and deformation following an external force. Also, we found that local changes in the stiffness can alter the cells response to external forces over long distances. Our results suggest that this long-range signaling of the substrate stiffness depends on the cells ability to resist deformation. Furthermore, we found that the cells elasticity in the apico-basal direction provides a level of detachment between the apical cell-cell junctions and the basal focal adhesions. Our simulation results show potential for increased ECM stiffness, e.g. due to a tumor, to modulate mechanical signaling between cells also outside the stiff region. Furthermore, the developed model provides a good platform for future studies on the interactions between epithelial monolayers and elastic substrates. Author summaryCells can communicate using mechanical forces, which is especially important in epithelial tissues where the cells are highly connected. Also, the stiffness of the material under the cells, called the extracellular matrix, is known to affect cell behavior, and an increase in this stiffness is related to many diseases, including cancers. However, it remains unclear how the stiffness affects intercellular mechanical signaling. We studied this effect using epithelial cells cultured on synthetic deformable substrates and developed a computational model to quantify the results better. In our experiments and simulations, we moved one cell to observe how the substrate stiffness impacts the deformation of the neighboring cells and thus the force transduction between the cells. Our model also enabled us to study the effect of local stiffness changes on the force transduction. Our results showed that substrate stiffness has an apparent impact on the force transduction within the epithelial tissues. Furthermore, we found that the cells can communicate information on the local stiffness changes over long distances. Therefore, our results indicate that the cellular mechanical signaling could be affected by changes in the substrate stiffness which may have a role in the progression of diseases such as cancer.