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bioRxiv · 10.1101/2021.09.03.458910

Dopamine-dependent cAMP dynamics in basal amygdala glutamatergic neurons

Lutas, A.·Fernando, K.·Zhang, S. X.·Sambangi, A.·Andermann, M. L.
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Abstract

Dopaminergic inputs to basal amygdala (BA) instruct learning of motivational salience. Here, we investigated the dynamics of dopamine release and downstream signaling during multiple salient events occurring within tens of seconds. We established in vitro and in vivo real-time tracking and manipulation of cAMP - a key intracellular plasticity signal downstream of dopamine receptor activation. Optogenetically-evoked release of dopamine drove proportional increases in cAMP in almost all BA glutamatergic neurons, suggesting widespread actions of dopamine across neurons preferring positive or negative valence. These cAMP responses decayed more slowly than dopamine release, potentially extending the window of plasticity. cAMP levels accumulated following direct photostimulation of cAMP but not repeated stimulation of dopamine axons, due to potent depression of dopamine release. cAMP and protein kinase A (PKA) responses to repeated appetitive or aversive stimuli also exhibited pronounced depression. Thus, history-dependent dynamics of dopamine and cAMP may regulate learning of temporally clustered, salient stimuli.

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Lutas, A., Fernando, K., Zhang, S. X., Sambangi, A., Andermann, M. L.. 2021-09-04. Dopamine-dependent cAMP dynamics in basal amygdala glutamatergic neurons. https://doi.org/10.1101/2021.09.03.458910

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