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bioRxiv · 10.1101/2021.09.03.458793

CRISPRs are associated with bacterial expansion in the human gut during the first year of life

Wu, J.·Barraclough, T. G.
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Abstract

The dynamics of the gut microbiome in infancy have a profound impact on health in adulthood, but the ecological mechanism underlying the dynamics between bacteria and bacteriophages remains poorly understood. CRISPR is a bacterial adaptive immune system to resist bacteriophages; however, the role they play in the dynamics in infants gut microbiota is unknown. In this work, using large-scale metagenomic sequencing data from 82 Sweden infants gut microbiomes, 1882 candidate CRISPRs were identified and their dynamics were analyzed. The results showed CRISPRs were distributed in dominant bacteria and could target distinct bacteriophages at different time points with largely alternated spacers. In the putative identical CRISPRs, we found the CRISPRs could acquire new spacers and loss old spacers during the first year. In addition, it is the first time to report that gender was a major factor to determine the bacterial richness and the number of CRISPRs and host range of bacteriophages was narrow in silico. Therefore, we concluded that CRISPRs were associated with bacterial expansion. This work improves the understanding of the ecological mechanism behind dynamics in the early life of the human gut and substantially expands the repertoire of predicted CRISPRs providing a resource to study the function of bacterial unknown genes and to enhance the performance of these beneficial bacteria by CRISPR gene-editing technology.

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Wu, J., Barraclough, T. G.. 2021-09-04. CRISPRs are associated with bacterial expansion in the human gut during the first year of life. https://doi.org/10.1101/2021.09.03.458793

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