M-CAMP™: A cloud-based web platform with a novel approach for species-level classification of 16S rRNA microbiome sequences

Schriefer, A. E.; Kumar, B.; Zolty, A.; R, P.; Didier, A.; MG, N.; Nadiv, N.; Perez, M.; Mahankuda, S. K.; Kumar, P.; GT, G.; Tenney, A.; Bourner, M.; Lezer, S.; Zhong, F.; Daniely, M.; Liu, Y.

doi:10.1101/2021.08.25.456838

bioRxiv · 10.1101/2021.08.25.456838

M-CAMP™: A cloud-based web platform with a novel approach for species-level classification of 16S rRNA microbiome sequences

Schriefer, A. E.·Kumar, B.·Zolty, A.·R, P.·Didier, A.·MG, N.·Nadiv, N.·Perez, M.·Mahankuda, S. K.·Kumar, P.·GT, G.·Tenney, A.·Bourner, M.·Lezer, S.·Zhong, F.·Daniely, M.·Liu, Y.

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Abstract

The M-CAMP (Microbiome Computational Analysis for Multiomic Profiling) Cloud Platform was designed to provide users with an easy-to-use web interface to access best in class microbiome analysis tools. This interface allows bench scientists to conduct bioinformatic analysis on their samples and then download publication-ready graphics and reports. The core pipeline of the platform is the 16S-seq taxonomic classification algorithm which provides species-level classification of Illumina 16s sequencing. This algorithm uses a novel approach combining alignment and kmer based taxonomic classification methodologies to produce a highly accurate and comprehensive profile. Additionally, a comprehensive proprietary database combining reference sequences from multiple sources was curated and contains 18056 unique V3-V4 sequences covering 11527 species. The M-CAMP 16S taxonomic classification algorithm was validated on 52 sequencing samples from both public and in-house standard sample mixtures with known fractions. Compared to current popular public classification algorithms, our classification algorithm provides the most accurate species-level classification of 16S rRNA sequencing data.

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Schriefer, A. E., Kumar, B., Zolty, A., R, P., Didier, A., MG, N., Nadiv, N., Perez, M., Mahankuda, S. K., Kumar, P., GT, G., Tenney, A., Bourner, M., Lezer, S., Zhong, F., Daniely, M., Liu, Y.. 2021-08-27. M-CAMP™: A cloud-based web platform with a novel approach for species-level classification of 16S rRNA microbiome sequences. https://doi.org/10.1101/2021.08.25.456838

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