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bioRxiv · 10.1101/2021.08.23.457392

Superovulation does not alter calcium oscillations following fertilization

Abstract

Superovulation is a common approach to maximize the number of eggs available for either clinical assisted reproductive technologies or experimental animal studies. This procedure provides supraphysiological amounts of gonadotropins to promote continued growth and maturation of ovarian follicles that otherwise would undergo atresia. There is evidence in mice, cows, sheep and humans that superovulation has a detrimental impact on the quality of the resulting ovulated eggs or embryos. Here we tested the hypothesis that eggs derived from superovulation have a reduced capacity to support calcium oscillations following fertilization, which is a critical factor in the success of embryo development. Eggs were obtained from mice that were either naturally cycling or underwent a standard superovulation protocol. Naturally cycling mice were mated to vasectomized males and vaginal plugs were checked to assure ovulation had occurred. The superovulated mice were also mated to vasectomized males for consistency of treatment across groups. The eggs were fertilized in vitro while undergoing monitoring of calcium oscillatory patterns. There were no differences in any measures of calcium oscillatory behavior, including length of the first oscillation, area under the curve of calcium signal, or frequency or persistence of oscillations. These findings indicate that superovulation does not disrupt calcium signaling at fertilization, supporting the use of this method for both clinical and experimental purposes.

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BibTeXRIS

Savy, V., Stein, P., Shi, M., Williams, C. J.. 2021-08-24. Superovulation does not alter calcium oscillations following fertilization. https://doi.org/10.1101/2021.08.23.457392

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