Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells.

Martini, V.; Edmans, M.; Gubbins, S.; Jayaraman, S.; Paudyal, B.; Morgan, S.; McNee, A.; Morin, T.; Rijal, P.; Gerner, W.; Sewell, A. K.; Inoue, R.; Bailey, M.; Connelley, T.; Charleston, B.; Townsend, A.; Beverley, P.; Tchilian, E.

doi:10.1101/2021.08.23.457377

bioRxiv · 10.1101/2021.08.23.457377

Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells.

Martini, V.·Edmans, M.·Gubbins, S.·Jayaraman, S.·Paudyal, B.·Morgan, S.·McNee, A.·Morin, T.·Rijal, P.·Gerner, W.·Sewell, A. K.·Inoue, R.·Bailey, M.·Connelley, T.·Charleston, B.·Townsend, A.·Beverley, P.·Tchilian, E.

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Abstract

We defined naive, central memory, effector memory and terminally differentiated porcine CD8 T cells and analyzed their phenotype in lymphoid and respiratory tissues after influenza infection or immunization using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM), that express CD69 and have an effector memory or terminally differentiated phenotype. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. The frequency of NP-specific cells declines over 63 days in all tissues but is best maintained in BAL. The pig is a powerful model for understanding how best to induce and harness local immunity to respiratory viruses. One sentence summaryInfluenza NP-specific porcine tissue resident memory CD8 T cells persist in the lung with major changes in gene expression.

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Martini, V., Edmans, M., Gubbins, S., Jayaraman, S., Paudyal, B., Morgan, S., McNee, A., Morin, T., Rijal, P., Gerner, W., Sewell, A. K., Inoue, R., Bailey, M., Connelley, T., Charleston, B., Townsend, A., Beverley, P., Tchilian, E.. 2021-08-23. Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells.. https://doi.org/10.1101/2021.08.23.457377

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