Acoustic light-sheet microscopy

Wunderl, S.; Ishijima, A.; Susaki, E.; Xu, Z.; Song, H.; Zha, H.; Azuma, T.; Sakuma, I.; Fukuoka, H.; Okada, E.; Ueda, H. R.; Takagi, S.; Nakagawa, K.

doi:10.1101/2021.08.20.457051

bioRxiv · 10.1101/2021.08.20.457051

Acoustic light-sheet microscopy

Wunderl, S.·Ishijima, A.·Susaki, E.·Xu, Z.·Song, H.·Zha, H.·Azuma, T.·Sakuma, I.·Fukuoka, H.·Okada, E.·Ueda, H. R.·Takagi, S.·Nakagawa, K.

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Abstract

Light-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light. We developed acoustic light-sheet microscopy (acoustic LSM), which actively manipulates the light propagation inside a large sample to obtain wide-field microscopic images deep inside a target. By accurately coupling a light-sheet illumination pulse into a planar acoustic pulse, the light-sheet can be continuously guided over large distances. We imaged a fluorescence-labeled transparent mouse brain for the FOVs of 19.3 x 12.4 mm2 and 9.7 x 5.9 mm2 with resolved microstructures and single cells deep inside the brain. Acoustic LSM creates new opportunities for the application of light-sheet in the field of industry to basic science. One Sentence SummaryAn acoustic-optical method overcomes a trade-off between field-of-view and axial resolution in light-sheet microscopy.

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Wunderl, S., Ishijima, A., Susaki, E., Xu, Z., Song, H., Zha, H., Azuma, T., Sakuma, I., Fukuoka, H., Okada, E., Ueda, H. R., Takagi, S., Nakagawa, K.. 2021-08-20. Acoustic light-sheet microscopy. https://doi.org/10.1101/2021.08.20.457051

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