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bioRxiv · 10.1101/2021.08.05.455081

Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survival

Abstract

Septic patients frequently develop skeletal muscle wasting and weakness, resulting in severe clinical consequences and adverse outcomes. Autophagy is a stress-induced degradative process essential to cell survival. Recent studies have demonstrated that sepsis triggers sustained induction of autophagy in skeletal muscles, although the impact of this enhanced autophagy on sepsis-induced muscle dysfunction remains unclear. Atg7 is an autophagy gene that plays a major role in autophagosome formation. Using an inducible and muscle-specific Atg7 knockout mouse model (Atg7iSkM-KO), we investigated the functional importance of skeletal muscle autophagy in sepsis. Sepsis was induced using cecal ligation and perforation (CLP) with a sham operation serving as a control. Atg7iSkM-KO mice exhibited a more severe phenotype in response to sepsis, marked by severe muscle wasting and contractile dysfunction, hypoglycemia, higher ketone levels and a decreased in survival as compared to mice with intact Atg7. Several genes that encode 26S proteasome subunits were upregulated, suggesting that activation of the ubiquitin-proteasome system is responsible for the severe muscle atrophy that was seen in these mice. Sepsis and Atg7 deletion resulted in the accumulation of mitochondrial dysfunction, although sepsis did not further worsen mitochondrial dysfunction in Atg7iSkM-KO mice. Overall, our study demonstrates that autophagy inactivation in skeletal muscles triggers significant worsening of sepsis-induced contractile and metabolic dysfunctions and negatively impacts survival. Induction of autophagy in skeletal muscles in response to sepsis thus represents a protective mechanism.

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BibTeXRIS

Leduc-Gaudet, J.-P., Miguez, K., Cefis, M., Moamer, A., Chaffer, T. J., Faitg, J., Reynaud, O., Broering, F. E., Shams, A., Mayaki, D., Huck, L., Sandri, M., Gouspillou, G., Hussain, S.. 2021-08-06. Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survival. https://doi.org/10.1101/2021.08.05.455081

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