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bioRxiv · 10.1101/2021.08.04.455135

Embedded 3D printing in self-healing annealable composites for functional patterning of human neural constructs

Kajtez, J.·Wessler, M. F.·Birtele, M.·Khorasgani, F. R.·Ottosson, D. R.·Heiskanen, A.·Kamperman, T.·Leijten, J.·Martinez-Serrano, A.·Larsen, N. B.·Angelini, T. E.·Parmar, M.·Lind, J. U.·Emneus, J.
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Abstract

Human in vitro models of neural tissue with controllable cellular identity, tunable microenvironment, and defined spatial arrangement are needed to facilitate studies of brain development and disease. Towards this end, embedded printing in jammed microgel supports (i.e., granular gels) holds great promise as it allows precise and programmable patterning of extremely soft and compliant tissue constructs. However, in contrast to the vast material landscape available for bulk hydrogels, granular printing support formulations are restricted to a handful of materials without the ability for facile adjustment of biofunctional properties of the cellular microenvironment. Therefore, there has been a need for novel materials that take advantage of versatile biomimicry of bulk hydrogels while providing high-fidelity support for embedded printing akin to granular gels. To address this need, we present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self-Healing Annealable Particle-Extracellular matrix (SHAPE) composites. SHAPE composites consist of soft microgels immersed in viscous extracellular-matrix solution to enable precise freeform patterning of human stem cells and consequent generation and long-term maintenance of mature subtype-specific neurons that extend projections within the volume of the annealed support. The developed approach further allows multi-ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular channels. Due to its modularity, SHAPE biomanufacturing toolbox not only offers a solution for functional modeling of mechanically sensitive neural constructs, but also has potential to be applied to a wide range of biomaterials with different crosslinking mechanisms to model tissues and diseases where recapitulation of complex architectural features and topological cues is essential.

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Kajtez, J., Wessler, M. F., Birtele, M., Khorasgani, F. R., Ottosson, D. R., Heiskanen, A., Kamperman, T., Leijten, J., Martinez-Serrano, A., Larsen, N. B., Angelini, T. E., Parmar, M., Lind, J. U., Emneus, J.. 2021-08-06. Embedded 3D printing in self-healing annealable composites for functional patterning of human neural constructs. https://doi.org/10.1101/2021.08.04.455135

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