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bioRxiv · 10.1101/2021.07.28.454054

Inferring cell-cell interactions from pseudotime ordering of scRNA-Seq data

Abstract

A major advantage of single cell RNA-Sequencing (scRNA-Seq) data is the ability to reconstruct continuous ordering and trajectories for cells. To date, such ordering was mainly used to group cells and to infer interactions within cells. Here we present TraSig, a computational method for improving the inference of cell-cell interactions in scRNA-Seq studies. Unlike prior methods that only focus on the average expression levels of genes in clusters or cell types, TraSig fully utilizes the dynamic information to identify significant ligand-receptor pairs with similar trajectories, which in turn are used to score interacting cell clusters. We applied TraSig to several scRNA-Seq datasets. As we show, using the ordering information allows TraSig to obtain unique predictions that improve upon those identified by prior methods. Functional experiments validate the ability of TraSig to identify novel signaling interactions that impact vascular development in liver organoid.

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BibTeXRIS

Li, D., Velazquez, J. J., Ding, J., Hislop, J., Ebrahimkhani, M. R., Bar-Joseph, Z.. 2021-07-29. Inferring cell-cell interactions from pseudotime ordering of scRNA-Seq data. https://doi.org/10.1101/2021.07.28.454054

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