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bioRxiv · 10.1101/2021.07.28.454029

Hormonal contraceptives alter amphetamine place preference and responsivity in the intact female rat.

Hilz, E. N.·Olvera, M.·Jun, D. S.·Chadha, M.·Gillette, R.·Monfils, M. H.·Gore, A. C.·Lee, H. J.
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Abstract

Hormonal contraceptives (HCs) are commonly used among reproductive aged women and alter the physiological state of the user by interfering with endogenous hormone concentrations and their actions on the reproductive tract. As hormones such as estradiol and progesterone modulate the incidence of substance abuse disorders in women, it is important to consider the influence HCs have on the female brain and behavior. This experiment explores how female sex steroid hormonal states associated with the rat estrous cycle, and modulating those states with HCs, influences measures of drug preference and responsivity. First, rats underwent food-light Pavlovian conditioning to measure conditioned orienting, a known predictor of amphetamine (AMP) place preference. Then, rats were conditioned and tested for AMP place preference with either an HC-implant or during estrous cycle stages associated with different ovarian hormone levels (i.e., proestrus (P) or metestrus/diestrus (M/D) while recording ultrasonic vocalizations (USVs) as an index of hedonic responsivity. Because of dopamines (DA) role in modulation of AMP actions, DA cell activity and availability were examined using tyrosine hydroxylase and FOS immunohistochemistry after final AMP challenge. Conditioned orienting did not differ between cycling and HC-implanted. P rats emitted more USVs during conditioning, showed higher AMP place preference throughout testing, and had higher DA cell activity in the substantia nigra compared to M/D and HC-implanted rats. Sex steroid hormone serum concentration and uterine horn thickness predicted some but not all of these measures. This experiment suggests ovarian hormones affect drug preference and responsivity, while providing novel insight into how hormone-altering contraceptives may reduce these measures.

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Hilz, E. N., Olvera, M., Jun, D. S., Chadha, M., Gillette, R., Monfils, M. H., Gore, A. C., Lee, H. J.. 2021-07-29. Hormonal contraceptives alter amphetamine place preference and responsivity in the intact female rat.. https://doi.org/10.1101/2021.07.28.454029

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