Green self-immolative polymer: molecular antenna to collect and propagate the signal for zymogen activation

Montasell, M. C.; Monge, P.; Carmali, S.; Mesquita, L.; Andersen, D.; Lovschall, K.; Sogaard, A.; Kristensen, M.; Pütz, J.; Zelikin, A. N.

doi:10.1101/2021.07.25.453687

bioRxiv · 10.1101/2021.07.25.453687

Green self-immolative polymer: molecular antenna to collect and propagate the signal for zymogen activation

Montasell, M. C.·Monge, P.·Carmali, S.·Mesquita, L.·Andersen, D.·Lovschall, K.·Sogaard, A.·Kristensen, M.·Pütz, J.·Zelikin, A. N.

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Abstract

Chemical zymogens of three different types were established herein around protein cysteinome, in each case converting the protein thiol into a disulfide linkage: zero length Z0, polyethylene glycol based ZPEG, and ZLA that features a fast-depolymerizing fuse polymer. The latter was a polydisulfide based on a naturally occurring water-soluble lipoic acid. Three zymogen designs were applied to cysteinyl proteases and a kinase and in each case, enzymatic activity was successfully masked in full and reactivated by small molecule reducing agents. However, only ZLA could be reactivated by protein activators, demonstrating that the macromolecular fuse escapes the steric bulk created by the protein globule, collects activation signal in solution, and relays it to the enzyme active site. This afforded first-in-class chemical zymogens that are activated via protein-protein interactions. For ZLA, we also document a "chain transfer" bioconjugation mechanism and a unique zymogen exchange reaction between two proteins.

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Montasell, M. C., Monge, P., Carmali, S., Mesquita, L., Andersen, D., Lovschall, K., Sogaard, A., Kristensen, M., Pütz, J., Zelikin, A. N.. 2021-07-25. Green self-immolative polymer: molecular antenna to collect and propagate the signal for zymogen activation. https://doi.org/10.1101/2021.07.25.453687

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