CFTR modulator response measurements in subjects with cystic fibrosis using 2D differentiated nasal epithelia converted into spheroids

Amatngalim, G. D.; Rodenburg, L. W.; Aalbers, B. L.; Raeven, H. H. M.; Aarts, E. M.; Silva, I. A. L.; Nijenhuis, W.; Vrendenbarg, S.; Kruisselbrink, E.; Brunsveld, J.; van Drunen, C. M.; Michel, S.; de Winter-de Groot, K. M.; Heijerman, H. G.; Kapitein, L. C.; Amaral, M. D.; van der Ent, C. K.; Beekman, J. M.

doi:10.1101/2021.07.20.453105

bioRxiv · 10.1101/2021.07.20.453105

CFTR modulator response measurements in subjects with cystic fibrosis using 2D differentiated nasal epithelia converted into spheroids

Amatngalim, G. D.·Rodenburg, L. W.·Aalbers, B. L.·Raeven, H. H. M.·Aarts, E. M.·Silva, I. A. L.·Nijenhuis, W.·Vrendenbarg, S.·Kruisselbrink, E.·Brunsveld, J.·van Drunen, C. M.·Michel, S.·de Winter-de Groot, K. M.·Heijerman, H. G.·Kapitein, L. C.·Amaral, M. D.·van der Ent, C. K.·Beekman, J. M.

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Abstract

Cystic Fibrosis (CF) is caused by genetic defects that impair the cystic fibrosis transmembrane conductance regulator (CFTR) channel in airway epithelial cells. These defects may be overcome by specific CFTR modulating drugs, for which the efficacy can be predicted in a personalized manner using 3D nasal-brushing-derived airway organoids in a forskolin-induced swelling assay. Despite of this, previously described application of 3D airway organoids in CFTR function assays have not been fully optimal. In this report we therefore describe an alternative method of culturing nasal brushing-derived airway organoids, which are created from an equally differentiated airway epithelial monolayer of a 2D air-liquid interface culture. In addition, we have defined organoid culture conditions, with the growth factor/cytokine combination neuregulin-1{beta} and interleukine-1{beta}, which enabled consistent detection of CFTR modulator responses in nasal airway organoids cultures from subjects with CF.

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Amatngalim, G. D., Rodenburg, L. W., Aalbers, B. L., Raeven, H. H. M., Aarts, E. M., Silva, I. A. L., Nijenhuis, W., Vrendenbarg, S., Kruisselbrink, E., Brunsveld, J., van Drunen, C. M., Michel, S., de Winter-de Groot, K. M., Heijerman, H. G., Kapitein, L. C., Amaral, M. D., van der Ent, C. K., Beekman, J. M.. 2021-07-21. CFTR modulator response measurements in subjects with cystic fibrosis using 2D differentiated nasal epithelia converted into spheroids. https://doi.org/10.1101/2021.07.20.453105

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