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bioRxiv · 10.1101/2021.07.12.452110

TOR signalling is required for host lipid metabolic remodelling and survival following enteric infection in Drosophila

Deshpande, R. S.·Lee, B.·Qiao, Y.·Grewal, S. S.
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Abstract

When infected by enteric pathogenic bacteria, animals need to initiate local and whole-body defence strategies. While most attention has focused on the role of innate immune anti-bacterial responses, less is known about how changes in host metabolism contribute to host defence. Using Drosophila as a model system, we identify induction of intestinal target-of-rapamycin (TOR) kinase signalling as a key adaptive metabolic response to enteric infection. We find TOR is induced independently of the IMD innate immune pathway, and functions together with IMD signalling to promote infection survival. These protective effects of TOR signalling are associated with re-modelling of host lipid metabolism. Thus, we see that TOR switches intestinal metabolism to lipolysis and fatty acid oxidation. In addition, TOR is required to limit excessive infection mediated wasting of adipose lipid stores by promoting an increase in the levels of fat body-expressed de novo lipid synthesis genes. Our data support a model in which induction of TOR represents a host tolerance response to counteract infection-mediated lipid wasting in order to promote survival.

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Deshpande, R. S., Lee, B., Qiao, Y., Grewal, S. S.. 2021-07-12. TOR signalling is required for host lipid metabolic remodelling and survival following enteric infection in Drosophila. https://doi.org/10.1101/2021.07.12.452110

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