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bioRxiv · 10.1101/2021.07.02.450857

Dasabuvir inhibits human norovirus infection in human intestinal enteroids

Hayashi, T.·Murakami, K.·Hirano, J.·Fujii, Y.·Yamaoka, Y.·Ohashi, H.·Watashi, K.·Estes, M. K.·Muramatsu, M.
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Abstract

Human noroviruses (HuNoVs) are acute viral gastroenteritis pathogens that affect all age groups, yet no approved vaccines and drugs to treat HuNoV infection are available. In this study, with a human intestinal enteroid (HIE) culture system where HuNoVs are able to replicate reproducibly, we screened an antiviral compound library to identify compound(s) showing anti-HuNoV activity. Dasabuvir, which has been developed as an anti-hepatitis C virus agent, was found to inhibit HuNoV infection in HIEs at micromolar concentrations. Dasabuvir also inhibited severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human A rotavirus (RVA) infection in HIEs. To our knowledge, this is the first study to screen an antiviral compound library for HuNoV using HIEs and we successfully identified dasabuvir as a novel anti-HuNoV inhibitor that warrants further investigation.

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Hayashi, T., Murakami, K., Hirano, J., Fujii, Y., Yamaoka, Y., Ohashi, H., Watashi, K., Estes, M. K., Muramatsu, M.. 2021-07-03. Dasabuvir inhibits human norovirus infection in human intestinal enteroids. https://doi.org/10.1101/2021.07.02.450857

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