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bioRxiv · 10.1101/2021.07.02.450806

Mapping Protein Numbers in Living Cells

Thirstrup, D. J.·Yao, J.·Sherman, J.·Mueller, I. A.·Wiegraebe, W.
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Abstract

We introduce a new, robust method to map the numbers of proteins in living cells. The method can be applied to laser scanning, spinning disk, and lattice light-sheet microscopes in a robust, reproducible, and scalable fashion. The method uses calibrated EGFP solutions that are imaged with the appropriate microscope modality to create a calibration curve that is then applied to convert the fluorescence intensities from 3D microscope images into molecule numbers. We applied this method to human induced pluripotent stem cells in which proteins representing key cellular structures were endogenously tagged with mEGFP. We used the ratio of mEGFP-tagged proteins to total proteins to create 3D maps of live cells showing the density of total proteins measured in molecules per {micro}m3. The method opens the door to new quantitative single cell analyses of cellular protein numbers in the context of single cell gene expression, associations with cellular complexes, and changes in cellular behaviors. The method is capable of quantifying protein numbers, over three orders of magnitude, in the cytoplasm or within various cellular structures while offering the unique advantages of each microscopy modality.

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Thirstrup, D. J., Yao, J., Sherman, J., Mueller, I. A., Wiegraebe, W.. 2021-07-03. Mapping Protein Numbers in Living Cells. https://doi.org/10.1101/2021.07.02.450806

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